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    Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)

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    To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.

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    Title : Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)
    Author(s) : Andrés Mauricio Posso Terranova1*, Heiber Cárdenas Henao2, Enrique Murgueitio3, Pascal Leterme1, y Jaime Eduardo Muoz Flórez1
    Abstract : To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.
    Keywords : DNA, DNA isolation, genetic markers, germplasm banks, nacedero, Trichanthera gigantea.

    Subject : unspecified
    Area : Computer Science
    Language : Spanish
    Year : 2011

    Affiliations Dirección Nacional de Bibliotecas - Universidad Nacional de Colombia
    University : Universidad Nacional de Colombia
    Journal : Acta Agronómica
    Volume : 60
    Url : http://www.revistas.unal.edu.co/index.php/acta_agronomica/article/view/27834/28082
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