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    block this user Werner Muller

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    Faculty of Life Science, University of Manchester, Manchester

    Mast cell-specific Cre/loxP-mediated recombination in vivo.

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    Mast cells are important effectors of type I allergy but also essential regulators of innate and adaptive immune responses. The aim of this study was to develop a Cre recombinase-expressing mouse line that allows mast cell-specific inactivation of genes in vivo. Following a BAC transgenic approach, Cre was expressed under the control of the mast cell protease (Mcpt) 5 promoter. Mcpt5-Cre transgenic mice were crossed to the ROSA26-EYFP Cre excision reporter strain. Efficient Cre-mediated recombination was observed in mast cells from the peritoneal cavity and the skin while only minimal reporter gene expression was detected outside the mast cell compartment. Our results show that the Mcpt5 promoter can drive Cre expression in a mast cell-specific fashion. We expect that our Mcpt5-Cre mice will be a useful tool for the investigation of mast cell biology.

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    Description

    Title : Mast cell-specific Cre/loxP-mediated recombination in vivo.
    Author(s) : Julia Scholten, Karin Hartmann, Alexander Gerbaulet, Thomas Krieg, Werner Müller, Giuseppe Testa, Axel Roers
    Abstract : Mast cells are important effectors of type I allergy but also essential regulators of innate and adaptive immune responses. The aim of this study was to develop a Cre recombinase-expressing mouse line that allows mast cell-specific inactivation of genes in vivo. Following a BAC transgenic approach, Cre was expressed under the control of the mast cell protease (Mcpt) 5 promoter. Mcpt5-Cre transgenic mice were crossed to the ROSA26-EYFP Cre excision reporter strain. Efficient Cre-mediated recombination was observed in mast cells from the peritoneal cavity and the skin while only minimal reporter gene expression was detected outside the mast cell compartment. Our results show that the Mcpt5 promoter can drive Cre expression in a mast cell-specific fashion. We expect that our Mcpt5-Cre mice will be a useful tool for the investigation of mast cell biology.
    Keywords : animals, bacterial proteins, bacterial proteins genetics, bacterial proteins metabolism, chymases, chymases genetics, chymases metabolism, dna primers, female, flow cytometry, integrases, integrases genetics, luminescent proteins, luminescent proteins gen

    Subject : unspecified
    Area : Other
    Language : English
    Year : 2008

    Affiliations Faculty of Life Science, University of Manchester, Manchester
    Journal : Transgenic Research
    Volume : 17
    Issue : 2
    Publisher : Springer
    Pages : 307-315
    Url : http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2268725&tool=pmcentrez&rendertype=abstract

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