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    block this user Werner Muller

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    Faculty of Life Science, University of Manchester, Manchester

    Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein.

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    Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked target sequences in the epidermis, in tongue, and thymic epithelium of the offspring where the Cre allele was inherited from the father. Where the mother carried the Cre allele, loxP flanked sequences were completely deleted in all tissues of the offspring, even in littermates that did not inherit the Cre allele. This maternally inherited phenotype indicates that the human K14 promoter is transcriptionally active in murine oocytes and that the enzyme remains active until after fertilization, even when the Cre allele becomes transmitted to the polar bodies during meiosis. Detection of K14 mRNA by RT-PCR in murine ovaries and immunohistochemical identification of the K14 protein in oocytes demonstrates that the human K14 promoter behaves like its murine homolog, thus identifying K14 as an authentic oocytic protein.

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    Description

    Title : Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein.
    Author(s) : Martin Hafner, Jutta Wenk, Arianna Nenci, Manolis Pasparakis, Karin Scharffetter-Kochanek, Neil Smyth, Thorsten Peters, Daniel Kess, Olaf Holtkötter, Pierre Shephard, Jeffrey E Kudlow, Hans Smola, Ingo Haase, Angela Schippers, Thomas Krieg, Werner M
    Abstract : Three mouse lines expressing Cre recombinase under the control of the human K14 promoter induced specific deletion of loxP flanked target sequences in the epidermis, in tongue, and thymic epithelium of the offspring where the Cre allele was inherited from the father. Where the mother carried the Cre allele, loxP flanked sequences were completely deleted in all tissues of the offspring, even in littermates that did not inherit the Cre allele. This maternally inherited phenotype indicates that the human K14 promoter is transcriptionally active in murine oocytes and that the enzyme remains active until after fertilization, even when the Cre allele becomes transmitted to the polar bodies during meiosis. Detection of K14 mRNA by RT-PCR in murine ovaries and immunohistochemical identification of the K14 protein in oocytes demonstrates that the human K14 promoter behaves like its murine homolog, thus identifying K14 as an authentic oocytic protein.
    Keywords : aging, aging physiology, animals, female, gene expression regulation, developmental, humans, immunohistochemistry, integrases, integrases genetics, integrases metabolism, keratin 14, keratins, keratins genetics, keratins metabolism, male, mice, transgenic

    Subject : unspecified
    Area : Other
    Language : English
    Year : 2004

    Affiliations Faculty of Life Science, University of Manchester, Manchester
    Journal : Genesis New York NY 2000
    Volume : 38
    Issue : 4
    Pages : 176-181
    Url : http://dx.doi.org/10.1002/gene.20016
    Doi : 10.1002/gene.20016

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