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    block this user Antonio Lourenco

    Student, Ph.D. Level

    UTL -Instituto Superior de Agronomia

    Evaluation of Methods To Assess the Biofilm-Forming Ability of Listeria monocytogenes

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    The contamination of ready-to-eat products with Listeria monocytogenes has been related to the presence of biofilms in production lines, as biofilms protect cells from chemical sanitizers. The ability of L. monocytogenes to produce biofilms is often evaluated using in vitro methodologies. This work aims to compare the most frequently used methodologies, including high-throughput screening methods based on microplates (crystal violet and the Calgary Biofilm Device) and methods based on CFU enumeration and microscopy after growth on stainless steel. Thirty isolates with diverse origins and genetic characteristics were evaluated. No (or low) correlations between methods were observed. The only significant correlation was found between the methods using stainless steel. No statistically significant correlation (P > 0.05) was detected among genetic lineage, serovar, and biofilm-forming ability. Because results indicate that biofilm formation is influenced by the surface material, the extrapolation of results from high-throughput methods using microplates to more industrially relevant surfaces should be undertaken with caution.

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    Description

    Title : Evaluation of Methods To Assess the Biofilm-Forming Ability of Listeria monocytogenes
    Author(s) : António Lourenço, Francisco Rego, Luisa Brito, Joseph F. Frank
    Abstract : The contamination of ready-to-eat products with Listeria monocytogenes has been related to the presence of biofilms in production lines, as biofilms protect cells from chemical sanitizers. The ability of L. monocytogenes to produce biofilms is often evaluated using in vitro methodologies. This work aims to compare the most frequently used methodologies, including high-throughput screening methods based on microplates (crystal violet and the Calgary Biofilm Device) and methods based on CFU enumeration and microscopy after growth on stainless steel. Thirty isolates with diverse origins and genetic characteristics were evaluated. No (or low) correlations between methods were observed. The only significant correlation was found between the methods using stainless steel. No statistically significant correlation (P > 0.05) was detected among genetic lineage, serovar, and biofilm-forming ability. Because results indicate that biofilm formation is influenced by the surface material, the extrapolation of results from high-throughput methods using microplates to more industrially relevant surfaces should be undertaken with caution.
    Subject : unspecified
    Area : Other
    Language : English
    Year : 2012

    Affiliations UTL -Instituto Superior de Agronomia
    Journal : Journal of Food Protection
    Volume : 75
    Issue : 8
    Pages : 1411 - 1417
    Url : http://openurl.ingenta.com/content/xref?genre=article&issn=0362-028X&volume=75&issue=8&spage=1411
    Doi : 10.4315/0362-028X.JFP-11-464

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