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    block this user Mahendra Kumar Trivedi

    Independent researcher / mahendra@trivedisrl.com

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus

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    Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stableexotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus(ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II wasfurther subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzedon day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into threeseparate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All theexperimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing wascarried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. Theantimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00%respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant(35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantiallychanged in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers werechanged in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that theidentified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, thenearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These resultssuggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.

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    Description

    Title : Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus
    Author(s) : Mahendra Kumar Trivedi,Alice Branton,Dahryn Trivedi,Gopal Nayak
    Abstract : Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stableexotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus(ATCC 25923) was divided into two parts, Group (Gr.) I: control and Gr. II: treatment. After biofield treatment, Gr. II wasfurther subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzedon day 159 after revival (Study I). The revived sample (Gr. IIB) were retreated on day 159 (Study II), and divided into threeseparate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All theexperimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing wascarried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. Theantimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00%respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant(35.71%) alteration in treated sample with respect to control. The biotype number and microbial species were substantiallychanged in Gr. IIA (767177; Staphylococcus cohnii subsp. urealyticum) on day 10, while only the biotype numbers werechanged in rest of the treated samples as compared to control (307016; S. aureus). The 16S rDNA analysis showed that theidentified strain in this experiment was S. aureus (GenBank Accession No.: L37597) after biofield treatment. However, thenearest homolog genus-species was found as Staphylococcus simiae (GenBank Accession No.: DQ127902). These resultssuggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.
    Keywords : Staphylococci, Staphylococcus aureus, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction,Biotype, 16S rDNA, Gram-Positive Bacteria

    Subject : microbiology
    Area : Open Science
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : American Journal of BioScience
    Volume : 3
    Issue : 6
    Publisher : science PG
    Doi : 10.11648/j.ajbio.20150306.13

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    Mahendra's Peer Evaluation activity

    Downloads 24829
    Views 162
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Junior professional, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX, Center for the Study of Interdisicplinarity, University of North Texas, Denton, TX, Eckerd College, St. Petersburg, FL.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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