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    block this user Mahendra Kumar Trivedi

    Independent researcher / mahendra@trivedisrl.com

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

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    Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current studywas attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well asrevived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilizedvial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was furthersubdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysedon day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes.First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters werestudied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated samplewas carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobialsusceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treatedcells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant tosusceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 μg/mL) by two-fold in all thetreated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration inthe treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA(53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus)on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with specieswere substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and(53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showedthat the identified microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. Thenearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). Theresults suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state.

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    Title : Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen
    Author(s) : Mahendra Kumar Trivedi
    Abstract : Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current studywas attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well asrevived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilizedvial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. II: treated. Gr. II was furthersubdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analysed on day 10 while Gr. IIB was stored and analysedon day 159 (Study I). After retreatment on day 159, the sample (Study II) was divided into three separate tubes.First, second and third tube was analysed on day 5, 10 and 15, respectively. All experimental parameters werestudied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing of lyophilized treated samplewas carried out to correlate the phylogenetic relationship of C. braakii with other bacterial species. The antimicrobialsusceptibility and minimum inhibitory concentration showed 39.29% and 15.63% alteration respectively in treatedcells of C. braakii as compared to control. Tetracycline showed improved sensitivity pattern, i.e., from resistant tosusceptible after biofield treatment, with support of decreased MIC value (>8 to ≤ 4 μg/mL) by two-fold in all thetreated samples as compared to the control. Biochemical reactions also showed significant (42.42%) alteration inthe treated samples with respect to the control. Biotype numbers with species were substantially changed in Gr. IIA(53131052, Citrobacter freundii complex) on day 10 and in Gr. IIB, Study I (53111052; Citrobacter amalonaticus)on day 159 as compared to the control (77365776; Citrobacter braakii). Moreover, biotype numbers with specieswere substantially changed in Gr. IIB, Study II after retreatment on day 5 (53111042, Citrobacter amalonaticus) and(53131052; Citrobacter freundii complex) on day 10 and 15 as compared to the control. 16S rDNA analysis showedthat the identified microbe as Citrobacter freundii (GenBank Accession Number: DQ517285) with 95% identity. Thenearest homolog genus-species of C. braakii was found to be Citrobacter werkmanii (Accession No. AF025373). Theresults suggested that biofield treatment has a significant impact on C. braakii in lyophilized as well as revived state.
    Keywords : Citrobacter braakii; Antimicrobial susceptibility; Biofieldtreatment; Biochemical reaction; Biotype; 16S rDNA analysis; Gramnegativebacteria

    Subject : microbiology
    Area : Open Access
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : Journal of Clinical & Medical Genomics
    Volume : 3
    Issue : 1
    Publisher : Omics
    Doi : 10.4172/jcmg.1000129

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    Mahendra's Peer Evaluation activity

    Downloads 23643
    Views 162
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Junior professional, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX, Center for the Study of Interdisicplinarity, University of North Texas, Denton, TX, Eckerd College, St. Petersburg, FL.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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