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    block this user Mahendra Kumar Trivedi

    Independent researcher

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment

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    Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. Thecurrent study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived stateof P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procuredfrom Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number andstored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P.vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIAand Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, thesample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. Allexperimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing oflyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial speciesafter treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alterationrespectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) werealtered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr.IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showedthat the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homologgenus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris inlyophilized as well as revived state.

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    Description

    Title : Phenotyping and Genotyping Characterization of Proteus vulgaris After Biofield Treatment
    Author(s) : Mahendra Kumar Trivedi
    Abstract : Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. Thecurrent study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived stateof P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procuredfrom Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number andstored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P.vulgaris were divided in two parts, Gr. I: control and Gr. II: treatment. Group II was further subdivided into two parts, Gr. IIAand Gr. IIB. Gr. IIA was analysed on day 10. Gr. IIB was stored and analysed on day 143. After retreatment on day 143, thesample was divided into three separate tubes. First, second and third tubes were analysed on day 5, 10 and 15 respectively. Allexperimental parameters were studied using automated Micro Scan Walk-Away® system. The 16S rDNA sequencing oflyophilized treated sample was carried out to correlate the phylogenetic relationship of P. vulgaris with other bacterial speciesafter treatment. The antimicrobial susceptibility and minimum inhibitory concentration showed 10.71% and 15.63% alterationrespectively in treated cells of P. vulgaris as compared to control. It was observed that few biochemical reactions (6%) werealtered in the treated groups with respect to control. Moreover, biotype number was substantially changed in treated cells, Gr.IIA (62060406, Proteus penneri) on day 10 as compared to control (62070406; Proteus vulgaris). 16S rDNA analysis showedthat the identified sample in this experiment was Proteus vulgaris after biofield treatment. However, the nearest homologgenus-species was found to be Proteus hauseri. The results suggested that biofield treatment has impact on P. vulgaris inlyophilized as well as revived state.
    Keywords : Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype,16S rDNA Analysis,TrivediEffect,The Trivedi Effect, Mahendra Kumar Trivedi,Mahendra Trivedi,Biofield Energy Treatment,Biofield

    Subject : microbiology
    Area : Open Access
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : Journal of Genetics and Genomics
    Volume : 3
    Issue : 6
    Publisher : Science PG
    Doi : 10.11648/j.ijgg.20150306.12

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    Mahendra's Peer Evaluation activity

    Downloads 38586
    Views 200
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Student, Ph.D. Level, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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