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    block this user Mahendra Kumar Trivedi

    Independent researcher

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium

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    Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS)has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr.Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’si.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment.The control and treated samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis indicated theincrease in crystallite size by 5.20% in treated PBS as compared to the control. Similarly, the treated HBS also showed increase incrystallite size by 3.20% with respect to the control. Additionally, the treated PBS showed an increase in Bragg’s angle (2θ) ascompared to the control sample. However, a decrease in Bragg’s angle of XRD peaks of the treated sample was noticed in thetreated HBS. The DSC analysis of the control PBS showed melting temperature at 224.84°C; however melting temperature wasnot observed in the treated sample. However, DSC analysis of the treated HBS showed an increase in melting temperature(152.83°C) in comparison with the control (150.60°C). Additionally, the latent heat of fusion of the treated HBS was increasedsubstantially by 108.83% as compared to the control. The TGA thermogram of the treated PBS showed an increase in onset ofthermal degradation (212°C) as compared to the control (199°C). Whereas, the treated HBS showed less weight loss comparingwith the control sample. This indicated the increase in thermal stability of the both the treated PBS and HBS samples. The FT-IRspectroscopic analysis of treated PBS showed alterations in the frequency of the functional groups such as O-H, C-H, P=O,O=P-OH, and P-OH as compared to the control. Additionally, the FT-IR spectrum of the treated HBS showed increase infrequency of calcium chloride phase (1444→1448 cm-1) as compared to the control sample. Altogether, it was observed thatbiofield energy treatment had caused physical, thermal and spectral changes in the treated samples as compared to the control. Itis assumed that biofield energy treated PBS and HBS could be a good prospect for biological and tissue culture applications.

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    Description

    Title : Comparative Physicochemical Evaluation of Biofield Treated Phosphate Buffer Saline and Hanks Balanced Salt Medium
    Author(s) : Mahendra Kumar Trivedi
    Abstract : Phosphate buffer saline (PBS) has numerous biological and pharmaceutical applications. Hank buffer salt (HBS)has been used as a medium for tissue culture applications. This research study was aimed to investigate the influence of Mr.Trivedi’s biofield energy treatment on physicochemical properties of the PBS and HBS. The study was executed in two group’si.e. control and treated. The control group was kept aside as control and treated group had received the biofield energy treatment.The control and treated samples were further characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. The XRD analysis indicated theincrease in crystallite size by 5.20% in treated PBS as compared to the control. Similarly, the treated HBS also showed increase incrystallite size by 3.20% with respect to the control. Additionally, the treated PBS showed an increase in Bragg’s angle (2θ) ascompared to the control sample. However, a decrease in Bragg’s angle of XRD peaks of the treated sample was noticed in thetreated HBS. The DSC analysis of the control PBS showed melting temperature at 224.84°C; however melting temperature wasnot observed in the treated sample. However, DSC analysis of the treated HBS showed an increase in melting temperature(152.83°C) in comparison with the control (150.60°C). Additionally, the latent heat of fusion of the treated HBS was increasedsubstantially by 108.83% as compared to the control. The TGA thermogram of the treated PBS showed an increase in onset ofthermal degradation (212°C) as compared to the control (199°C). Whereas, the treated HBS showed less weight loss comparingwith the control sample. This indicated the increase in thermal stability of the both the treated PBS and HBS samples. The FT-IRspectroscopic analysis of treated PBS showed alterations in the frequency of the functional groups such as O-H, C-H, P=O,O=P-OH, and P-OH as compared to the control. Additionally, the FT-IR spectrum of the treated HBS showed increase infrequency of calcium chloride phase (1444→1448 cm-1) as compared to the control sample. Altogether, it was observed thatbiofield energy treatment had caused physical, thermal and spectral changes in the treated samples as compared to the control. Itis assumed that biofield energy treated PBS and HBS could be a good prospect for biological and tissue culture applications.
    Keywords : Trivedi Effect,The Trivedi Effect, Mahendra Kumar Trivedi,Mahendra Trivedi,Biofield Energy Treatment,Phosphate Buffer Saline, Hank Buffer Salt

    Subject : pharmaceutical
    Area : Open Access
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : American Journal of BioScience
    Volume : 3
    Issue : 6
    Publisher : Science PG
    Url : http://trivediscience.com/publications/pharmaceuticals-publications/comparative-physicochemical-evaluation-of-biofield-treated-phosphate-buffer-saline-and-hanks-balanced-salt-medium/
    Doi : 10.11648/j.ajbio.20150306.20

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    Mahendra's Peer Evaluation activity

    Downloads 38383
    Views 200
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Student, Ph.D. Level, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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