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    block this user Mahendra Kumar Trivedi

    Independent researcher / mahendra@trivedisrl.com

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium

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    Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue cultureapplications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment onphysicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The controlgroup was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treatedsamples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer and surface areaanalyzer. The XRD analysis revealed the amorphous nature of the control and treated peptone samples. The DSC analysis showedan increase in thermal denaturation temperature of the treated peptone (196.22°C) as compared to the control sample (141.20°C).Additionally, the exothermic peak of treated sample (280°C) was increased as compared to the control (270°C). The DSC ofcontrol and treated MMTO showed the absence of the melting temperature in their respective DSC thermograms. The TGAanalysis of the treated peptone showed an increase in onset of thermal degradation (172°C) with respect to the control (170°C).Nevertheless, the TGA thermogram of the treated MMTO (293.96°C) showed an increase in maximum thermal degradationtemperature (Tmax) as compared with the control (281.41°C). It indicated the good thermal stability of the treated peptone andMMTO samples. The FT-IR result of the treated peptone showed an upward shift in C-H (2817→2833 cm-1), and amide I(1635→1641 cm-1), stretching in the treated sample with respect to the control sample. Whereas, the FT-IR spectrum of thetreated MMTO showed an increase in the frequency of the C-H (2817→2833 cm-1) and amide I (1596→1606 cm-1) bands ascompared to the control. Particle size analysis of the treated peptone showed an increase in d50 (average particle size) and d99 (sizeexhibited by 99% of particles) by 9.3 and 41.4%, respectively with respect to the control. Surface area analysis showed increase insurface area by 4.3% in the treated peptone. Altogether, the results corroborated that the biofield energy treatment had altered thephysical, thermal and spectral properties of peptone and MMTO. It is assumed that biofield treated peptone and MMTO could beutilized as potential candidates for cell culture applications.

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    Description

    Title : Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium
    Author(s) : Mahendra Kumar Trivedi
    Abstract : Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue cultureapplications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment onphysicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The controlgroup was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treatedsamples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer and surface areaanalyzer. The XRD analysis revealed the amorphous nature of the control and treated peptone samples. The DSC analysis showedan increase in thermal denaturation temperature of the treated peptone (196.22°C) as compared to the control sample (141.20°C).Additionally, the exothermic peak of treated sample (280°C) was increased as compared to the control (270°C). The DSC ofcontrol and treated MMTO showed the absence of the melting temperature in their respective DSC thermograms. The TGAanalysis of the treated peptone showed an increase in onset of thermal degradation (172°C) with respect to the control (170°C).Nevertheless, the TGA thermogram of the treated MMTO (293.96°C) showed an increase in maximum thermal degradationtemperature (Tmax) as compared with the control (281.41°C). It indicated the good thermal stability of the treated peptone andMMTO samples. The FT-IR result of the treated peptone showed an upward shift in C-H (2817→2833 cm-1), and amide I(1635→1641 cm-1), stretching in the treated sample with respect to the control sample. Whereas, the FT-IR spectrum of thetreated MMTO showed an increase in the frequency of the C-H (2817→2833 cm-1) and amide I (1596→1606 cm-1) bands ascompared to the control. Particle size analysis of the treated peptone showed an increase in d50 (average particle size) and d99 (sizeexhibited by 99% of particles) by 9.3 and 41.4%, respectively with respect to the control. Surface area analysis showed increase insurface area by 4.3% in the treated peptone. Altogether, the results corroborated that the biofield energy treatment had altered thephysical, thermal and spectral properties of peptone and MMTO. It is assumed that biofield treated peptone and MMTO could beutilized as potential candidates for cell culture applications.
    Keywords : Peptone,Thermal Analysis,evaluation of peptone,ftir spectrum of peptone,Trivedi Effect,The Trivedi Effect, Mahendra Kumar Trivedi,Mahendra Trivedi,Biofield Energy Treatment

    Subject : pharmaceutical
    Area : Open Access
    Language : English
    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : American Journal of Bioscience and Bioengineering
    Volume : 3
    Issue : 6
    Publisher : Science PG
    Url : http://trivediscience.com/publications/pharmaceuticals-publications/physicochemical-evaluation-of-biofield-treated-peptone-and-malmgren-modified-terrestrial-orchid-medium/
    Doi : 10.11648/j.bio.20150306.15

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    Mahendra's Peer Evaluation activity

    Downloads 26069
    Views 162
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Junior professional, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX, Center for the Study of Interdisicplinarity, University of North Texas, Denton, TX, Eckerd College, St. Petersburg, FL.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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