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    This was brought to you by:

    block this user Mahendra Kumar Trivedi

    Independent researcher / mahendra@trivedisrl.com

    Las Vegas Naveda
    Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd

    Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media

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    The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (Tonset) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, Tmax (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165→3130 cm-1 (aromatic C-H stretching); 2813→2775 cm-1 (aliphatic C-H stretching); 1145→1137 cm-1 (C-N stretching), 995→1001 cm-1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (λmax) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.

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    Description

    Title : Physical, Thermal, and Spectroscopic Characterization of Biofield Energy Treated Murashige and Skoog Plant Cell Culture Media
    Author(s) : Mahendra Kumar Trivedi
    Abstract : The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Afterward, both the control and treated samples were analyzed using various analytical techniques. The X-ray diffraction (XRD) analysis showed 19.92% decrease in the crystallite size of treated sample with respect to the control. The thermogravimetric analysis (TGA) showed the increase in onset temperature of thermal degradation (Tonset) by 9.41% and 10.69% in first and second steps of thermal degradation, respectively after the biofield energy treatment as compared to the control. Likewise, Tmax (maximum thermal degradation temperature) was increased by 17.43% and 28.61% correspondingly in the first and second step of thermal degradation in the treated sample as compared to the control. The differential scanning calorimetry (DSC) analysis indicated the 143.51% increase in the latent heat of fusion of the treated sample with respect to the control sample. The Fourier transform infrared spectroscopy (FT-IR) spectrum of treated MS media showed the alteration in the frequency such as 3165→3130 cm-1 (aromatic C-H stretching); 2813→2775 cm-1 (aliphatic C-H stretching); 1145→1137 cm-1 (C-N stretching), 995→1001 cm-1 (S=O stretching), etc. in the treated sample with respect to the control. The UV spectra of control and treated MS media showed the similar absorbance maxima (λmax) i.e. at 201 and 198 nm, respectively. The XRD, TGA-DTG, DSC, and FT-IR results suggested that Mr. Trivedi’s biofield energy treatment has the impact on physical, thermal, and spectral properties of the MS media. As a result, the treated MS media could be more stable than the control, and might be used as better media in the plant tissue culture technique.
    Keywords : Murashige and Skoog Medium,X-ray Diffraction,Trivedi Effect,The Trivedi Effect, Mahendra Kumar Trivedi,Mahendra Trivedi,Biofield Energy Treatment,DSC of murashige and skoog

    Subject : pharmaceutical
    Area : Open Access
    Language : English
    Year : 2015

    Affiliations Trivedi Global Inc.
    Trivedi Science Research Laboratory Pvt. Ltd
    Journal : Cell Biology
    Volume : 3
    Issue : 4
    Publisher : Science PG
    Doi : 10.11648/j.cb.20150304.11

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    Mahendra's Peer Evaluation activity

    Downloads 23879
    Views 162
    Following... 21
    • Alejandro Engelmann, Independent researcher, Library, Swedish University of Agricultural Sciences, Uppsala, Sweden.
    • Selma Dorrestein, Student, Master Level, University of Amsterdam.
    • Francisco Herrera, Publisher, UNIVERSITY OF GRANADA.
    • Ralf Steinmetz, Professor, university.
    • Gregory Dudek, Professor, McGill University, School of Computer Science, Montreal, Canada.
    • Umberto Straccia, Senior Research Fellow, ISTI - CNR.
    • Sorin Cotofana, Associate Professor, Deft University of Technology, Faculty of Electrical Engineeting, Mathematics, and Computer Science. Computer Engineering, Delft, The Netherlands.
    • Stefan Trausan-Matu, Professor, Computer Science Department, Politehnica University of Bucharest, Research Institute for Artificial Intelligence.
    • Jean Quisquater, Professor, UCL Crypto Group.
    • Markus Jakobsson, Principal Research Fellow, PayPal, FatSkunk, Indiana University.
    • Michael Elad, Professor, Technion - Israel institute of Technology.
    • Andrew Lumsdaine, Professor, Indiana University.
    • Mikael Nilsson, Student, Ph.D. Level, Royal Institute of Technology, Stockholm, Sweden.
    • Emilie Combet, Lecturer, MVLS, University of Glasgow, Glasgow, Centre for Population and Health Sciences, Life-course Nutrition and Health.
    • Werner Muller, Professor, Faculty of Life Science, University of Manchester, Manchester.
    • Syam Mohan, Senior Research Fellow, Pharmacology, University of Malaya, Malaysia.
    • Ramy K Aziz, Lecturer, Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
    • Paweł K. Jędrzejko, Associate Professor, Department of American and Canadian Studies of the Institute of English Cultures and Literatures, University of Silesia in Katowice, Poland.
    • Nader Ale Ebrahim, Independent researcher, Research Support Unit, Centre of Research Services, Institute of Research Management and Monitoring (IPPP), University of Malaya, Malaysia.
    • Kelli Barr, Junior professional, Department of Philosophy and Religion Studies, University of North Texas, Denton, TX, Center for the Study of Interdisicplinarity, University of North Texas, Denton, TX, Eckerd College, St. Petersburg, FL.
    • Pandelis Perakakis, Post Doctorate, Economics department, Universitet Jaume I, Castellon.

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